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p chk2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p chk2
    Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.
    P Chk2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 545 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "SKA2 promotes gastric cancer progression by regulating glutathione metabolism"

    Article Title: SKA2 promotes gastric cancer progression by regulating glutathione metabolism

    Journal: iScience

    doi: 10.1016/j.isci.2026.115202

    Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.
    Figure Legend Snippet: Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.

    Techniques Used: Western Blot, Expressing, Knockdown, Over Expression, Control

    Related Articles

    Western Blot:

    Article Title: Altered MDC1 Interactions and Dysfunctional DNA Repair in Lobular Breast Cancer Confers Sensitivity to PARP Inhibition
    Article Snippet: .. Primary antibodies for immunoblots were: phospho-Histone H2A.X (Ser139)(20E3) (Cell Signaling Technology #9718; RRID:AB_2118009); ATM (D2E2) (Cell Signaling Technology #2873; RRID:AB_2062659); Phospho-ATM (Ser1981)(D6H9)( Cell Signaling Technology #5883; RRID:AB_10835213); MRE11 (31H4)(Cell Signaling Technology #4847; RRID:AB_10693469); phospho-MRE11 (Ser676)(Cell Signaling Technology #4859; RRID:AB_2145096); p95/NBS1 (NBN) (D6J5I)(Cell Signaling Technology #14956; RRID:AB_2798660); phospho-p95/NBS1 (NBN)(Ser343)(Cell Signaling Technology #3001; RRID:AB_10829154); Rad50 (Cell Signaling Technology #3427; RRID:AB_2176936); phospho-CHK1 (Ser345)(133D3)(Cell Signaling Technology #2348; RRID:AB_331212); phospho-CHK2 (Thr68)(C13C1)(Cell Signaling Technology #2197; RRID:AB_2080501); 53BP1 (E7N5D) XP (Cell Signaling Technology #88439; RRID:AB_3095656); phospho-53BP1 (Ser1618)(D4H11)(Cell Signaling Technology #6209; RRID:AB_11220229); phospho-53BP1 (Thr543)(Cell Signaling Technology #3428; RRID:AB_2206631); RAD51 (D4B10)(Cell Signaling Technology #8875; RRID:AB_2721109); DNA-PKcs (3H6)(Cell Signaling Technology #12311; RRID:AB_2797881); phospho-DNA-PKcs (Ser2056)(E9J4G)(Cell Signaling Technology #68716; RRID:AB_2939025); α/β-Tubulin (Cell Signaling Technology #2148; RRID:AB_2288042); Vinculin (E1E9V) XP (Cell Signaling Technology #13901; RRID:AB_2728768); MDC1 (MDC1–50)(Sigma-Aldrich M2444; RRID:AB_532268). .. Secondary antibodies for immunoblots were: HRP monoclonal mouse anti-rabbit light chain specific (JacksonImmunoResearch 211-032-171; RRID:AB_2339149); HRP goat anti-mouse light chain specific (115-035-174; RRID:AB_2338512); HRP-donkey anti-mouse (715-035-150; RRID:AB_2340770); and HRP donkey anti-rabbit (711-035-152; RRID:AB_10015282).

    Article Title: Circular RNA circATM binds PARP1 to suppress Wnt/β-catenin signaling and induce cell cycle arrest in gastric cancer cells
    Article Snippet: The following antibodies were used in the Co-IP assay: anti-FLAG M2 antibody (Sigma-Aldrich Cat# F1804, RRID:AB_262044), anti-PARP1 antibody (Proteintech Cat# 66520-1-Ig, RRID:AB_2881883), anti-β-catenin antibody (Cell Signaling Technology Cat# 8480, RRID:AB_11127855), rabbit IgG (Cell Signaling Technology Cat# 3900, RRID:AB_1550038), and mouse IgG (Cell Signaling Technology Cat# 5415, RRID:AB_10829607). .. Antibodies targeting the following markers were used for western blotting: PARP (Cell Signaling Technology Cat# 9542, RRID:AB_2160739), Survivin (Cell Signaling Technology Cat# 2808, RRID:AB_2063948), Cox2 (Cell Signaling Technology Cat# 4842, RRID:AB_2084968), APC (Cell Signaling Technology Cat# 2504, RRID:AB_2057484), AXIN2 (Cell Signaling Technology Cat# 5863, RRID:AB_10835211), TCF4 (Cell Signaling Technology Cat# 2953, RRID:AB_2199265), p16 (Cell Signaling Technology Cat# 92803, RRID:AB_2750891), p21 (Cell Signaling Technology Cat# 2947, RRID:AB_823586), p27 (Cell Signaling Technology Cat# 3686, RRID:AB_2077850), CDK2 (Cell Signaling Technology Cat# 2546, RRID:AB_2276129), cyclin A2 (Cell Signaling Technology Cat# 4656, RRID:AB_2071958), ATM (Cell Signaling Technology Cat# 2873, RRID:AB_2062659), phospho-ATM (Ser1,981) (Cell Signaling Technology Cat# 4526, RRID:AB_2062663), Chk1 (Cell Signaling Technology Cat# 2360, RRID:AB_2080320), phospho-Chk1 (Cell Signaling Technology Cat# 2348, RRID:AB_331212), Chk2 (Cell Signaling Technology Cat# 6334, RRID:AB_11178526), phospho-Chk2 (Cell Signaling Technology Cat# 2197, RRID:AB_2080501), GAPDH (Cell Signaling Technology Cat# 5174, RRID:AB_10622025), cyclin D1, c-Jun, c-Myc, c-Met in the Wnt/β-catenin Activated Targets Antibody Sampler Kit (#8655), and γH2AX (S139) (Abcam Cat# ab81299, RRID:AB_1640564). .. Total RNA was extracted from the GC samples using the Eastep TM Super Total RNA Extraction Kit (Cat. LS1040, Promega, Beijing, China).

    Article Title: Selective targeting of apoptosis proteins by structurally-stabilized and/or cysteine-reactive NOXA peptides
    Article Snippet: .. Samples were subjected to electrophoresis and western analysis using 1:1000 dilutions of phospho-ATM (S1981, Cell Signaling, #4526), ATM (Abcam, #ab78), phospho-CHK2 (Ser33/35, Cell Signaling, #2665), CHK2 (Cell Signaling, #2662), and actin (Sigma #A1978) antibodies. ..

    other:

    Article Title: GSK3i combinatorial treatments affect CDK4/6 and compensatory pathways in 3D preclinical models of pancreatic neuroendocrine tumors
    Article Snippet: phospho-Chk2 , 2197, Cell Signaling Technology (Danvers, MA, USA) , 1:200.

    Article Title: TTI1 contributes to radioresistance by activating ATM pathway in rectal cancer.
    Article Snippet: Antibodies against Bcl-2(D17C4)(3498), Bax(D2E11) (5023), Chk2(D9C6)(6334), Phospho-Chk2(Thr68) (C13C1)(2197) and pan-Acetylated-Lysine(9441) were purchased from Cell Signaling Technology.

    Electrophoresis:

    Article Title: Selective targeting of apoptosis proteins by structurally-stabilized and/or cysteine-reactive NOXA peptides
    Article Snippet: .. Samples were subjected to electrophoresis and western analysis using 1:1000 dilutions of phospho-ATM (S1981, Cell Signaling, #4526), ATM (Abcam, #ab78), phospho-CHK2 (Ser33/35, Cell Signaling, #2665), CHK2 (Cell Signaling, #2662), and actin (Sigma #A1978) antibodies. ..



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    Cell Signaling Technology Inc phospho chk2
    Immunohistochemistry was performed for <t>phospho-Chk2</t> (A), phospho-H2AX (B) and phospho-Chk1 (C). Nuclear indices were calculated for phospho-Chk2 and phspho-H2AX. Scale bars: 20 µm; statistical analysis performed with ANOVA with Dunnett's multiple comparison to control. * P <0.05, ** P <0.01, **** P <0.0001.
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    Immunohistochemistry was performed for <t>phospho-Chk2</t> (A), phospho-H2AX (B) and phospho-Chk1 (C). Nuclear indices were calculated for phospho-Chk2 and phspho-H2AX. Scale bars: 20 µm; statistical analysis performed with ANOVA with Dunnett's multiple comparison to control. * P <0.05, ** P <0.01, **** P <0.0001.
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    phospho chk2 thr68  (Cell Signaling Technology Inc)
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    Immunohistochemistry was performed for <t>phospho-Chk2</t> (A), phospho-H2AX (B) and phospho-Chk1 (C). Nuclear indices were calculated for phospho-Chk2 and phspho-H2AX. Scale bars: 20 µm; statistical analysis performed with ANOVA with Dunnett's multiple comparison to control. * P <0.05, ** P <0.01, **** P <0.0001.
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    Image Search Results


    Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.

    Journal: iScience

    Article Title: SKA2 promotes gastric cancer progression by regulating glutathione metabolism

    doi: 10.1016/j.isci.2026.115202

    Figure Lengend Snippet: Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.

    Article Snippet: p-chk2 , Cell Signaling Technology , Cat# 2197; RRID: AB_2080501.

    Techniques: Western Blot, Expressing, Knockdown, Over Expression, Control

    Immunohistochemistry was performed for phospho-Chk2 (A), phospho-H2AX (B) and phospho-Chk1 (C). Nuclear indices were calculated for phospho-Chk2 and phspho-H2AX. Scale bars: 20 µm; statistical analysis performed with ANOVA with Dunnett's multiple comparison to control. * P <0.05, ** P <0.01, **** P <0.0001.

    Journal: Biology Open

    Article Title: GSK3i combinatorial treatments affect CDK4/6 and compensatory pathways in 3D preclinical models of pancreatic neuroendocrine tumors

    doi: 10.1242/bio.062358

    Figure Lengend Snippet: Immunohistochemistry was performed for phospho-Chk2 (A), phospho-H2AX (B) and phospho-Chk1 (C). Nuclear indices were calculated for phospho-Chk2 and phspho-H2AX. Scale bars: 20 µm; statistical analysis performed with ANOVA with Dunnett's multiple comparison to control. * P <0.05, ** P <0.01, **** P <0.0001.

    Article Snippet: phospho-Chk2 , 2197, Cell Signaling Technology (Danvers, MA, USA) , 1:200.

    Techniques: Immunohistochemistry, Comparison, Control